Abstract
Willow (Salix spp.) is gaining an increasing interest as a fast-growing tree with high biomass yield from low agricultural inputs, which contains potentially bioactive compounds. The present work aimed to develop a high-yield extraction procedure combined with robust, sensitive and fast microLiquid Chromatography-Triple Quadrupole Mass Spectrometry (LC-MS/MS) based method for comprehensively quantifying flavonoids and salicylic acid in the bark of Salix spp. We have investigated the effect of freeze- and oven-drying procedures and five extraction solvents on the yield of individual flavonoid and salicylic acid when performing classical solid-liquid extraction. The freeze-drying was the best drying procedure for preserving monomeric and polymeric flavan-3-ols, whereas other flavonoids were less affected. Salicylic acid was not affected by the drying procedures. The best extraction solvent in terms of the yield of individual flavonoid among the tested solvents in this study was the combination of methanol acidified with 1% hydrochloric acid. LC-MS/MS method has shown a high recovery percentage (≥80%), good precision and overall robustness.
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