Comprehensive proteomics and functional annotation of mouse brown adipose tissue.

Jing Li,Quan Liu,Zheng-Guang Guo,Yong Fu,Xiao-Yan Liu,Tao Yuan,Zhu-Fang She,Xiao-Yue Tang,Wei-Gang Zhao,Shuai-Nan Liu,Wei Sun,Hai-Dan Sun,Juan Li,Jonathan M Peterson

doi:10.1371/journal.pone.0232084

Abstract

Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.

Highlights

Two types of adipocytes exist in mammals, white and brown adipocytes [1]
To construct a comprehensive dataset for the mouse brown adipose tissue (BAT) proteome, proteins were extracted from interscapular BAT harvested from C57BL/6J female mice
The pooled digested peptides were separated into 60 fractions by reversed-phase liquid chromatography (RPLC), mixed into 20 fractions, and analyzed by nanoRPLC–MS/MS

Summary

Introduction

Two types of adipocytes exist in mammals, white and brown adipocytes [1]. White adipocytes are the major constituents of white adipose tissue (WAT), which contains energy-dense triglycerides that store energy [2]. Brown adipocytes are the major constituents of brown adipose tissue (BAT), which is situated in the interscapular, cervical, mediastinal and retroperitoneal regions [3, 4]. BAT plays a prominent role in thermogenesis and energy expenditure [5].

Methods

Results

Conclusion