Abstract
Recent exploitation of the avian immune system has highlighted its suitability for the generation of high-quality, high-affinity antibodies to a wide range of antigens for a number of therapeutic and biotechnological applications. The glycosylation profile of potential immunoglobulin therapeutics is species specific and is heavily influenced by the cell-line/culture conditions used for production. Hence, knowledge of the carbohydrate moieties present on immunoglobulins is essential as certain glycan structures can adversely impact their physicochemical and biological properties. This study describes the detailed N-glycan profile of IgY polyclonal antibodies from the serum of leghorn chickens using a fully quantitative high-throughput N-glycan analysis approach, based on ultra-performance liquid chromatography (UPLC) separation of released glycans. Structural assignments revealed serum IgY to contain complex bi-, tri- and tetra-antennary glycans with or without core fucose and bisects, hybrid and high mannose glycans. High sialic acid content was also observed, with the presence of rare sialic acid structures, likely polysialic acids. It is concluded that IgY is heavily decorated with complex glycans; however, no known non-human or immunogenic glycans were identified. Thus, IgY is a potentially promising candidate for immunoglobulin-based therapies for the treatment of various infectious diseases.
Highlights
Antibodies are at the forefront of the field of targeted therapeutics and diagnostics due to their natural high affinity and excellent half-life properties [1]
This study describes the detailed N-glycan profile of IgY polyclonal antibodies from the serum of Leghorn chickens using a fully quantitative high-throughput N-glycan analysis based on ultra-performance liquid chromatography (UPLC) separation of released glycans
IgY was successfully recovered from the serum of chickens using thiophilic adsorption, which is based on the principles of hydrophobic interaction chromatography
Summary
Antibodies are at the forefront of the field of targeted therapeutics and diagnostics due to their natural high affinity and excellent half-life properties [1]. PLOS ONE | DOI:10.1371/journal.pone.0159859 July 26, 2016
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
