Abstract
Blood stasis syndrome (BSS) is one of the most common Chinese medicine patterns in coronary heart disease. Our previous work proved that Xueshuan Xinmaining Tablet (XXT) could treat blood stasis through regulating the expression of F13a1, Car1 and Tbxa2r. In the current study, the effect and mechanism of XXT on BSS was comprehensively and holistically investigated based on a metabolomics approach. Urine and plasma samples of 10 BBS rats treated with XXT (XT), 9 BSS model rats (BM) and 11 normal control (NC) rats were collected and then determined by UPLC-Q/TOP-MS. Multivariate analyses were applied to distinguish differentiate urinary and plasma metabolite patterns between three groups. Results showed that a clear separation of three groups was achieved. XT group was located between BM group and NC group, and showing a tendency of recovering to NC group, which was consistent with the results of hemorheological studies. Some significantly changed metabolites like cortexolone, 3α,21-dihydroxy-5β-pregnane-11,20-dione and 19S-hete and leukotriene A4, chiefly involved in steroid hormone biosynthesis, arachidonic acid metabolism and lipid metabolism, were found and identified to explain the mechanism. These potential markers and their corresponding pathways will help explain the mechanism of BSS and XXT treatment. This work also proves that metabolomics is effective in traditional Chinese medicinal research.
Highlights
IntroductionBlood stasis syndrome (BSS), a slowing or pooling of blood [1], is one of the most common
Blood stasis syndrome (BSS), a slowing or pooling of blood [1], is one of the most commonChinese medicine patterns in coronary heart disease [2]
According to traditional Chinese medicines (TCMs) theory, BSS is mainly triggered by abnormalities of blood flow and viscosity which are initially induced by anger emotions and exogenous pathogenic factor such as a cold environment
Summary
Blood stasis syndrome (BSS), a slowing or pooling of blood [1], is one of the most common. Chinese medicine patterns in coronary heart disease [2]. It plays a significant role in the development of multiple disorders and diseases [3,4]. Hemorheology is an important evaluation index to estimate blood stasis [5,6]. Xueshuan Xinmaining Tablet (XXT) formula, widely used in the treatment of cardiovascular and cerebrovascular diseases in China, is composed of ten traditional Chinese medicines (TCMs) including. XXT was reported to significantly ameliorate the syndrome and exhibit protective activities via decreasing hemorheological parameters
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