Abstract
White adipose tissue (WAT) plays a significant role in energy metabolism and the obesity epidemic. In this study, we sought to (1) profile the mouse WAT proteome with advanced 2DLC/MS/MS approach, (2) provide insight into WAT function based on protein functional annotation, and (3) predict potentially secreted proteins. A label-free 2DLC/MS/MS proteomic approach was used to identify the WAT proteome from female mouse WAT. A total of 6,039 proteins in WAT were identified, among which 5,160 were quantified (spanning a magnitude of 106) using an intensity-based absolute quantification algorithm, and 3,117 proteins were reported by proteomics technology for the first time in WAT. To comprehensively analyze the function of WAT, the proteins were divided into three quantiles based on abundance and we found that proteins of different abundance performed different functions. High-abundance proteins (the top 90%, 1,219 proteins) were involved in energy metabolism; middle-abundance proteins (90–99%, 2,273 proteins) were involved in the regulation of protein synthesis; and low-abundance proteins (99–100%, 1,668 proteins) were associated with lipid metabolism and WAT beiging. Furthermore, 800 proteins were predicted by SignalP4.0 to have signal peptides, 265 proteins had never been reported, and five have been reported as adipokines. The above results provide a large dataset of the normal mouse WAT proteome, which might be useful for WAT function research.
Highlights
Mammals have two types of adipose tissue, brown adipose tissue (BAT) and white adipose tissue (WAT)
Our data provide a comprehensive mouse WAT proteome dataset, which might be useful for WAT function research
Workflow of proteome analysis In this study, samples of WAT from six C57BL/6J female mice were used to generate a comprehensive profile of the normal mouse WAT proteome
Summary
Mammals have two types of adipose tissue, brown adipose tissue (BAT) and white adipose tissue (WAT). Comprehensive map and functional annotation of the mouse white adipose tissue proteome. White adipose tissue secrete hormones and cytokines to regulate energy intake, energy expenditure, and carbohydrate and lipid metabolism, e.g., adiponectin (ADI), leptin, and plasminogen activator inhibitor-1 (McGown, Birerdinc & Younossi, 2014). ADI, one of the most abundant adipokines produced by adipocytes, plays a fundamental role in maintaining vascular homeostasis, protecting from vascular injury and atherogenesis (Vaiopoulos et al, 2012; Nishimura et al, 2008). These proteins are considered a valuable resource in disease research. A comprehensive profile of the WAT proteome will provide a basic understanding of WAT function and benefit disease studies
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