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Abstract
The pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profiles of the anterior pituitary of estrus and anestrus sheep using RNA-seq technology. Through this study, we identified a total of 12,468 circRNAs and 9231 differentially expressed circRNAs in the estrus and anestrus pituitary system of sheep. We analyzed some differentially expressed circRNAs by reverse transcription quantitative-PCR (RT-qPCR), and some circRNAs were demonstrated using RNase-R+ resistance experiments. CircRNAs involving the regulation of estrus-related terms and pathways are enriched by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In addition, we also predicted partial microRNA-circRNA interaction network for circRNAs that regulate sheep estrus. Overall, this study explored a potential substantial role played by circRNAs involved in pituitary regulation on sheep estrus and proposed new questions for further study.
Highlights
Most sheep are seasonal estrus animals, and the reproductive rate of sheep is closely related to the time and frequency of estrus [1]
More than 210 million raw reads were detected in the pituitary glands of estrus and anestrus sheep
Abundance and differences of circular RNA (circRNA) in the pituitary gland during estrus and anestrus in sheep, we used the FindCirc low quality reads, and these clean reads were used for the bioinformatics analysis
Summary
Most sheep are seasonal estrus animals, and the reproductive rate of sheep is closely related to the time and frequency of estrus [1]. Previous studies have shown that there are significant physiological and endocrinological differences in the pituitary, when comparing estrous cycling ewes with anestrus ewes [2]. References have shown that these reproductive-related hormones’ secretion levels are different between the estrus and anestrus periods of the ewes. Zhang et al showed that the levels of hormones such as progesterone, estrogen, LH and FSH were higher in the estrus phase of Kazakh sheep [5]. Most of these hormones are proteins and are primarily regulated in the synthesis and secretion at the transcriptional and post-transcriptional levels, which leads us to take into account
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