Comprehensive Evaluation and Physiological Response of Quinoa Genotypes to Low Nitrogen

Abstract

The utilization of low-N tolerant and N-efficifent varieties offers significant benefits in terms of reducing the need for excessive N fertilizer input. Quinoa, a resilient crop for agroecological transition, possesses a wide genetic diversity, making it suitable for selecting genotypes that require less N fertilizer. In this study, the growth and physiological characteristics of nine quinoa genotypes were assessed to determine their low-N tolerance using the fuzzy membership function. Based on comprehensive evaluation indices, three genotypes were identified: low-N tolerant (BL22), intermediately tolerant (A29), and sensitive (G68). These genotypes were exposed to varying N concentrations, including normal (4 mM), low (0.8 mM), and deficient N (no N) conditions. The results indicate that low-N conditions altered root phenotype, with reduced biomass, total protein, and chlorophyll content; increased soluble sugar levels; and inhibited N-metabolizing enzymes (nitrate reductase, glutamine synthetase, glutamate synthase) and N uptake. Under low-N conditions, the tolerant genotype exhibited higher maximal efficiency of photosystem II (Fv/Fm), root vitality, and N content compared to the sensitive genotype. Interestingly, the sensitive genotype displayed elongated and thinner shoots and roots in response to low-N, suggesting that plant height and root length are unreliable indicators of low-N tolerance in quinoa. In contrast, shoot and root dry biomass, Fv/Fm, chlorophyll content, N-metabolizing enzymes, and N content proved to be reliable indicators of low-N tolerance during the early growth stage of quinoa. Overall, these findings highlight the potential of utilizing specific growth and physiological parameters as indicators for screening low-N tolerant quinoa genotypes, thereby reducing dependence on N fertilizers.