Abstract
Sebastiscus species, marine rockfishes, are of essential economic value. However, the genomic data of this genus is lacking and incomplete. Here, whole genome sequencing of all species of Sebastiscus was conducted to provide fundamental genomic information. The genome sizes were estimated to be 802.49 Mb (S. albofasciatus), 786.79 Mb (S. tertius), and 776.00 Mb (S. marmoratus) by using k-mer analyses. The draft genome sequences were initially assembled, and genome-wide microsatellite motifs were identified. The heterozygosity, repeat ratios, and numbers of microsatellite motifs all suggested possibly that S. tertius is more closely related to S. albofasciatus than S. marmoratus at the genetic level. Moreover, the complete mitochondrial genome sequences were assembled from the whole genome data and the phylogenetic analyses genetically supported the validation of Sebastiscus species. This study provides an important genome resource for further studies of Sebastiscus species.
Highlights
The genus Sebastiscus is an interesting and commercial rockfish that mainly distributes in the Western Pacific [1]
A total of 40.14 Gb (S. albofasciatus, sequencing depth ~50×), 41.93 Gb (S. tertius, sequencing depth ~53×), and 87.55 Gb (S. marmoratus, sequencing depth ~112×) clean data were generated by whole genome sequencing
The first genome survey study of all Sebastiscus species was performed based on whole genome sequencing data of three different individuals
Summary
The genus Sebastiscus is an interesting and commercial rockfish that mainly distributes in the Western Pacific [1]. As the next-generation, high-throughput sequencing (NGS) has been developing rapidly over recent decades, the whole genome sequencing (WGS) and various molecular genetic tools have become efficient strategies for generating genomic resources and widely applied in genomic and evolutionary studies of marine fish species [9,10,11,12,13]. Compare the genomes of different Sebastiscus species, complete genome data were obtained by NGS and used to assemble the genome, estimate genome size, identify simple sequence repeats (SSRs), and extract the mitogenome. These data will be the basis of a fundamental genomic resource for extending our current knowledge of Sebastiscus genome organization and difference. These data provide a foundation for future genomic studies of Sebastiscus species
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