Abstract
Lipids play an important role in platelet integrity and function. However, most of lipid molecular compositions in platelets still remain unknown, especially for phospholipids. In this work, we characterized detailed structures of different classes of phospholipids in human platelets. Using a flow microreactor, the acetone Paterno-Buchi reaction was introduced for online derivatization of carbon–carbon double bond (C=C) of lipids right after separation by liquid chromatography (LC). Tandem mass spectrometry (MS/MS) methods were developed for multi-level structural identification of phospholipids, including head group, chain composition, and C=C locations in the fatty acyl/alkyl chains. After optimization of the workflow, a total of 110 molecular species of phospholipids were identified in the human platelet samples, including many C=C location isomers. At lipid class level, phosphatidylserine was found more abundant in the platelet samples than that of the plasma samples; at isomer level, the relative ratio of C18:1 (Δ9) vs C18:1 (Δ11) also increased in platelet samples comparing with that of the plasma samples. The detailed structural information of phospholipids and their C=C isomers ratios would be useful for investigation of potential functions of human platelets potentially associated with phospholipid isomers.
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