Abstract
Lignans are known to be an important class of phenylpropanoid secondary metabolites. In the course of our studies on the chemodiversity of lignans, the necessity arose to develop a method for the fast detection and identification of bioactive lignan subclasses. In this study, we detected 10 lignan derivatives of different extracts of F. viridissima by UHPLC-ESI-QTOF-MS. Lignan glycosides (1 and 2), lignans (3 and 4), and lignan dimers (5–10) were identified by analysis of their exact masses and MSe spectra along with the characteristic mass fragmentation patterns and molecular formulas. We further investigated NO inhibitory effects of F. viridissima fractions and their major lignan derivatives to evaluate those anti-inflammatory effects. The methylene chloride fraction of F. viridissima as well as compounds 8 and 10 showed potent dose-dependent NO inhibitory effects on RAW 264.7 cells. Corresponding to the NO inhibition by compounds 8 and 10, lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression was notably reduced by both compounds. Our combined data with the bioactive results and the component analysis by UHPLC-ESI-QTOF-MS suggest that the methylene chloride fraction of F. viridissima roots could be potential anti-inflammatory agents and these are related to major lignans including dimeric dibenzylbutyrolactone lignans.
Highlights
Lipopolysaccharide (LPS), the major cell wall component of gram-negative bacteria, induces inflammatory responses when administered to cells or animals
Enhanced production of inflammatory mediators is important for host defense against external stimuli including LPS, excess production of inflammatory mediators causes severe inflammatory diseases, including septic shock, rheumatoid arthritis, systemic lupus erythematosus (SLE), and inflammatory bowel disease (IBD) [5,6,7]
Based on our previous research, this study aimed to investigate the profiles of the lignan dimers, the profiles of the lignan dimers, lignans, and lignan glycosides with the help of a mass spectrometric lignans, and lignan glycosides with the help of a mass spectrometric technique and to assess the technique and to assess the anti-inflammatory activities of isolated compounds of roots of F
Summary
Lipopolysaccharide (LPS), the major cell wall component of gram-negative bacteria, induces inflammatory responses when administered to cells or animals. It induces the production of inflammatory mediators, including nitric oxide (NO), prostaglandin E2 , and proinflammatory cytokines [1,2,3,4]. 22 of evaluation for the development of new anti-inflammatory drugs due to the severe adverse effects of developed, various natural products and those components are under evaluation for the development NSAIDs [8]. Of new anti-inflammatory drugs due to the severe adverse effects of NSAIDs [8]. Arctigenin is known as an indicator component for the the Korean Pharmacopoeia (KP11). Lignan is one of the representative secondary metabolite class in F
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
