Comprehensive Assessment of Intergeneric Cross-compatibility of Six Fruit Tree Species in the Tribe Maleae (Rosaceae) Based on <i>In Vivo</i> Pollen Tube Growth and Field Pollination

Abstract

The development of intergeneric hybrids for horticultural crops has been attempted to introduce new quality and resistance traits and to enlarge the gene pool. Interspecific and intergeneric hybridization are often hindered by incompatibility reactions occurring at various stages of hybridization, from early pollination to initial growth, and the reproductive stages of the progeny. In this study, we investigated intergeneric and interspecific cross-compatibility among six species in the tribe Maleae (Rosaceae), namely, Pyrus communis (European pear), P. pyrifolia (Japanese pear), Malus × domestica (apple), Eriobotrya japonica (loquat), Cydonia oblonga (quince), and Pseudocydonia sinensis (Chinese quince). In vivo pollen tube growth tests showed the presence of a postmating, prezygotic barrier in many cross-combinations, in which cross-compatibility was regulated by both genetic distance and crossing direction. Strong hybridization barriers were observed in pollen tube growth and fruit setting when intergeneric hybridization was performed with E. japonica, a species phylogenetically distant from the others studied. Different compatibility reactions in reciprocal crosses were observed in some intergeneric hybridizations; C. oblonga as a pollen donor was incompatible with P. sinensis, whereas the reciprocal cross was compatible, resulting in the development of hybrid seedlings. Furthermore, the pollen tube growth rate differed among Pyrus species when pollinated on the apple pistils, suggesting divergence of cross-compatibility response in a specific linage. Factors affecting intergeneric hybridization are discussed with reference to the genetic distance between species and morphological characteristics such as pistil length. Our comprehensive assessment of intergeneric cross-compatibility will help provide a way to overcome crossing barriers and develop new hybrid crops in the tribe Maleae.