Comprehensive and efficient analyses of T cell receptors for cancer antigens in peptide-vaccinated patients using a single cell-based gene cloning system

Hidetoshi Nakagawa,Atsushi Muraguchi,Eishiro Mizukoshi,Hiroyuki Kishi,Shuichi Kaneko,Eiji Kobayashi

doi:10.1186/2051-1426-2-s3-p137

Abstract

Obtaining T cell receptor repertoires specific for cancer antigens in individuals is of great significance in terms of their relationships with the clinical courses and therapeutic application such as adoptive transfer. However, full analyses of T cell receptor (TCR) sequences have been difficult because of the inefficiency of T cell cloning, heterogeneity of T cells or the heterodimeric structures of TCRs. Here, we revealed complete landscapes of cancer antigen-specific TCRs using a novel TCR cloning system; hTEC10 (human TCR efficient cloning within 10 days) [1].

Highlights

Obtaining T cell receptor repertoires specific for cancer antigens in individuals is of great significance in terms of their relationships with the clinical courses and therapeutic application such as adoptive transfer
Fifteen patients were chosen from the participants of the Phase I clinical trial using HLA-A*2402-restricted a-fetoprotein (AFP)-derived peptide vaccine for advanced hepatocellular carcinoma (HCC)
347 specific T cell receptor (TCR) clones that consisted of 10 kinds of TCR gene rearrangements (3 from the CR patient; 4 from the long SD patient; 2 from the SD patient and 1 from the PD patient) were amplified

Summary

Introduction

Comprehensive and efficient analyses of T cell receptors for cancer antigens in peptidevaccinated patients using a single cell-based gene cloning system Introduction Obtaining T cell receptor repertoires specific for cancer antigens in individuals is of great significance in terms of their relationships with the clinical courses and therapeutic application such as adoptive transfer.

Results

Conclusion