Abstract
Colorectal cancer (CRC) has the fourth-highest incidence of all cancer types, and its incidence has steadily increased in the last decade. The general transcription factor III (GTF3) family, comprising GTF3A, GTF3B, GTF3C1, and GTFC2, were stated to be linked with the expansion of different types of cancers; however, their messenger (m)RNA expressions and prognostic values in colorectal cancer need to be further investigated. To study the transcriptomic expression levels of GTF3 gene members in colorectal cancer in both cancerous tissues and cell lines, we first performed high-throughput screening using the Oncomine, GEPIA, and CCLE databases. We then applied the Prognoscan database to query correlations of their mRNA expressions with the disease-specific survival (DSS), overall survival (OS), and disease-free survival (DFS) status of the colorectal cancer patient. Furthermore, proteomics expressions of GTF3 family members in clinical colorectal cancer specimens were also examined using the Human Protein Atlas. Finally, genomic alterations of GTF3 family gene expressions in colorectal cancer and their signal transduction pathways were studied using cBioPortal, ClueGO, CluePedia, and MetaCore platform. Our findings revealed that GTF3 family members’ expressions were significantly correlated with the cell cycle, oxidative stress, WNT/β-catenin signaling, Rho GTPases, and G-protein-coupled receptors (GPCRs). Clinically, high GTF3A and GTF3B expressions were significantly correlated with poor prognoses in colorectal cancer patients. Collectively, our study declares that GTF3A was overexpressed in cancer tissues and cell lines, particularly colorectal cancer, and it could possibly step in as a potential prognostic biomarker.
Highlights
According to global cancer statistics, colorectal cancer (CRC) causes more than700,000 deaths every year, and there will be an estimated 53,200 CRC deaths in UnitedStates in 2020 [1,2]
The results showed that CRC cell lines, such as HT29, SW48, and COLO320, exhibited significantly high expression levels of GTF3A, GTF3B, GTF3C1, and GTF3C2 (Figure 2A–E)
Database was used to plot a heatmap for all rows representing colorectal cancer cells based on expression levels of GTF3 family genes
Summary
According to global cancer statistics, colorectal cancer (CRC) causes more than. States in 2020 [1,2]. This evidence makes CRC one of the deadliest cancer types, along with lung cancer, liver cancer, and stomach cancer. The incidence has slightly increased year to year as globalization trends have grown in developing countries [3]. The number of cancer patients is increasing due to population growth and aging [5]. Researchers demonstrated rapid advances in the field of cancer therapy, where genetic alterations and the dysfunction of signal transduction pathways play important roles in cancer development [6,7,8]. Novel and effective therapeutic treatments and drug development are crucial [9,10,11]
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