Abstract
The MYB transcription factors (TFs) is a plant TF families, which involves in hormone signal transduction, and abiotic stress tolerance, etc. However, there are few studies on the MYB TFs family and its regulatory mechanism in Tamarix hispida. In this study, 14 MYB genes (named ThMYB1 - ThMYB14) were cloned and characterized from T. hispida. The transcription profiles of ThMYBs in T. hispida under different abiotic stress conditions were monitored using qRT-PCR. Most of studied ThMYBs were significantly downregulated and/or upregulated by salt and osmotic stress, ABA, GA3 and JA treatments in at least one organ. Especially, ThMYB13 was induced in the leaves and roots of T. hispida when exposed to NaCl treatment at all study periods, indicating that it may involve in salt stress. To further study ThMYB13 function, ThMYB13 overexpression and knock-down plants and control plants transformed with an empty pROKII were obtained using a transient transformation system. Overexpression of ThMYB13 in T. hispida displayed the lowest O2-, H2O2 and MDA accumulation, minimal cell death, the most stable K+/Na+ ratio and the lowest electrolyte leakage rate among the three kinds of transient expression in T. hispida. Conversely, the RNAi-silencing, transiently transformed plants displayed the opposite physiological changes. Therefore, ThMYB13 might play a role in salt stress tolerance in transgenic T. hispida plants.
Highlights
The MYB transcription factors (TFs) is most abundant in plants (Peng et al, 2016), which contains the MYB domain serving as DNA binding
The CDS of ThMYB13 was inserted into pROKII driving by 35S CaMV promoter to overexpress ThMYB13 (35S:MYB)
3 types of transiently transformed plantlets were cultured, including plants transiently transformed with 35S:MYB to overexpress ThMYB13 (OE), with pFGC:MYB for silencing ThMYB13 (RNAi) expression, or control (Con) plants transformed with the empty pROKII plasmid
Summary
The MYB TFs is most abundant in plants (Peng et al, 2016), which contains the MYB domain serving as DNA binding. The MYB family genes, R2R3 MYBs, were involved in diverse processes, including cell cycle control, hormone signaling, secondary metabolism, meristem formation and cellular morphogenesis. Some MYB genes were found to regulate responses to abiotic. AtMYB96 induced pathogen resistance through the pathway of ABA signaling that regulating stomatal movement, increased tolerance to drought and disease (Seo et al, 2009; Seo and Park, 2010). The silencing of GbMYB5 reduced antioxidant enzyme activities and proline content, leading to decreased the drought tolerance. Overexpression of GbMYB5 in tobacco improved tolerance to drought accompanied with decreased water loss, elevated the proline level and ROS scanvenging activities; the expression of SOD, CAT, GST, SAMDC and ADC1 were significantly induced (Chen et al, 2015)
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