Abstract
Studies related to miRNAs and their ability to tune protein expression have gained much attention in recent years. In the field of glycobiology, miRNAs are now given special consideration as they have been shown to be key regulators of many glycosylation pathways. The enzyme MGAT3 (β‐1,4‐mannosyl‐glycoprotein 4‐beta‐N‐acetylglucosaminyltransferase) is responsible for the addition of a bisecting GlcNAc to the core mannose residue of complex or hybrid N‐glycans. This is a unique modification of complex N‐glycans that has been reported to play important roles in signal transduction, growth factor signaling, tumor progression and metastasis. In this project, we generated a comprehensive map of the regulation of MGAT3 by miRNA using a newly developed high throughput assay: miRFluR. This assay uses ratiometric analysis of a genetically encoded dual‐color fluorescence reporter (cerulean/mCherry) to identify regulatory miRNA:mRNA interactions. A dataset for the interaction between the entire human miRome (~2700 miRs) and MGAT3 has been collected and analysis of the miR regulatory map is presented. Our data provides insight into the regulation of this important glycan and its functions in disease.
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