Abstract
Exosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the major miRNAs and proteins extracted from the exosomes were similar among the four cell lines. However, the miRNA profiles differed among the exosomes of each cell line, which corresponded to the expression patterns of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.
Highlights
Exosomes are small extracellular vesicles released from almost all cell types, including immune cells and tumour cells [1], as the intracellular endosome component
We considered that comprehensive analyses of both miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines could provide useful insights for understanding the molecular profiles and biological functions of exosomes derived from human lymphoid tumours
The RNA integrity numbers (RINs) and size distributions of total RNA samples taken from exosomes and parent cells are shown in S2 Fig. there were common peaks corresponding to ribosomal RNAs in exosomal RNA of the four cell lines, the distributions of RNA sizes were clearly different between exosomes and parent cells
Summary
Exosomes are small extracellular vesicles released from almost all cell types, including immune cells and tumour cells [1], as the intracellular endosome component. Exosomes were initially considered cellular waste, they have been shown to contain various molecules from the original cells, including proteins, functional mRNAs and miRNAs, and deliver these biological messages into the recipient cells [1,2]. Profiles of miRNA and protein within exosomes derived from lymphoid tumour cells role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Anicom Specialty Medical Institute Inc. provided support in the form of salaries for authors [T.U. and G.I.], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section
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