Comprehensive Analysis of MicroRNA and mRNA Expression in Normal and Tumorous Human Esophageal Squamous Cell Lines Using Microarray Datasets

Ichiro Akagi,Tsutomu Nomura,Hiroshi Yoshida,Nobutoshi Hagiwara,Osamu Ishibashi,Eiji Uchida,Hiroshi Makino,Akihisa Matsuda,Masao Miyashita,Takeshi Matsutani

doi:10.1155/2014/376541

Abstract

Despite the undisputed importance of altered microRNA (miRNA) expression in various cancers, there is limited information on the clinicopathologic significance of cancer‐related miRNAs in esophageal squamous cell carcinoma (ESCC). Previously, it was reported that the expression of several miRNAs was dysregulated in ESCC. However, the target genes of these miRNAs have not been identified. Furthermore, additional miRNAs in humans have been discovered recently, indicating that revised miRNA and gene expression profiling for ESCC are necessary. Here, we provide datasets from microarray analyses to identify miRNA and mRNA expression comprehensively in Het‐1A, a normal human esophageal squamous cell line, and three human ESCC cell lines.

Highlights

Esophageal cancer (EC) is the sixth most common cancer in the world that predominantly affects men and has a poor prognosis [1]
EC associated with distant metastasis and local invasion still leads to a poor prognosis
The RNA samples isolated from Het-1A and the esophageal squamous cell carcinoma (ESCC) cells were subjected to microarray-based expression analyses for mRNA using a SurePrint G3 Human Gene Expression 8 × 60 K v2 Array (Agilent)

Summary

Introduction

Esophageal cancer (EC) is the sixth most common cancer in the world that predominantly affects men and has a poor prognosis [1]. Efforts to identify the actual target mRNAs of miRNAs experimentally are necessary to understand how miRNAs are associated with the onset and progression of various diseases, including cancer, possibly leading to improvements in current diagnostic and prognostic indicators. To achieve this goal, we used microarray analysis, which is a powerful tool because it can analyze the expression of a number of mRNAs and miRNAs in multiple samples at once. We present the datasets from our microarray experiments to analyze comprehensively and comparably mRNA and miRNA expression in normal and tumorous human esophageal squamous cell lines

Methodology

Dataset Description

Concluding Remarks

Dataset Availability