Comprehensive analysis of lncRNA‑miRNA‑mRNA regulatory networks for Alzheimer's disease.

Abstract

Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative disease, associated with a decreased cognitive function and severe behavioral abnormalities. This study aimed to explore mechanisms of development and progression of AD. Comprehensive analysis of GSE16759 was performed to identify the differentially expressed lncRNAs (DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs). The differentially expressed RNAs (DERs) were used for the subsequent analysis, including module genes analysis, pathway enrichment analysis, and interaction network analysis. Finally, an AD‑associated network consisting of lncRNA‑miRNA‑mRNA‑pathway was constructed. A total of 431 DEmRNAs, 35 DElncRNAs, and 103 DEmiRNAs between the AD group and the normal control group were identified. DEmRNAs were significantly enriched in 13 pathways, such as focal adhesion, endocytosis, and mTOR signaling pathway. Three modules significantly related to AD were finally screened. The AD‑associated network was constructed, including 2 lncRNAs (A2M‑AS1 and ZNF571‑AS1), 1 miRNA (hsa‑miR‑206), 2 mRNAs (NOTCH3 and JAG1), and 2 pathways (notch signaling pathway and endocrine resistance). A2M‑AS1, ZNF571‑AS1, hsa‑miR‑206, NOTCH3 and JAG1 may be involved in the mechanisms of AD through notch signaling pathway and endocrine resistance.