Comprehensive analysis of driver mutations in Chinese squamous cell lung carcinomas by targeted next-generation sequencing.

Abstract

7544 Background: The aim of the study was to analyze the driver mutation profiles in a large cohort of Chinese SqCLCs to identify potential therapeutic targets. Methods: We detected approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes on 159 samples by using Ion Torrent semiconductor-based next-generation sequencing.We conducted FISH for fibroblast growth factor receptor 1 (FGFR1) and cyclin D1 (CCND1) amplification on 172 and 246 samples, respectively. In addtion, we analyzed PTEN expression by immunohistochemistry on 172 samples. Results: Somatic mutations were detected in 73.6% (117/159) of patients. The most commonly mutated gene detected in this study was TP53 (89 cases, 56.0%), followed by CDKN2A (14 cases, 8.8%), PI3KCA (14 cases, 8.8%), KRAS (7 cases, 4.4%), and EGFR (5 cases, 3.1%). The incidences of FGFR1 amplification, CCND1 amplification and loss of PTEN expression in this cohort of patients were 16.9% (29/172), 11% (27/246) and 43.6% (75/172), respectively. The fr...