Comprehensive analysis of B and T cell receptor repertoire in patients after kidney transplantation by high-throughput sequencing

Abstract

PurposeThe dynamic immunity of kidney transplant patients has not been fully elucidated. In this study, we explored the repertoire features of B/T cell receptor (BCR/TCR) of kidney transplant patients. MethodsUsing combined multiplex PCR amplification and high-throughput sequencing technique, we analyzed the uremic patients' BCR H chain and TCR beta chain repertoire which obtained 1 day before kidney transplantation (PRE-1), 1 day and 7 day after kidney transplantation (POST-1 and POST-7). ResultsOur analysis results showed the diversity of TCRβ CDR3 in POST-7 group was highest. In addition, there were specific skewed usage of TRBV gene subfamilies, and V-J combinations in different time points during kidney transplantation. Moreover, the overlap degrees of BCR-H (TCR-β) CDR3 repertoire among each group were identified. Notably, the abundance of some TCR-β CDR3 sequences changed regularly in the time point of kidney transplantation. ConclusionsThe BCR-H (TCR-β) CDR3 repertoire of kidney transplant patients changed dynamically.