Comprehensive Analysis of Antioxidant Compounds from Lippia citriodora and Hibiscus sabdariffa Green Extracts Attained by Response Surface Methodology.

María Del Carmen Villegas-Aguilar,Francisco Javier Leyva-Jiménez,Antonio Segura-Carretero,María De La Luz Cádiz-Gurrea,David Arráez-Román

doi:10.3390/antiox9121175

María Del Carmen Villegas-Aguilar, Francisco Javier Leyva-Jiménez + Show 3 more

Open Access

https://doi.org/10.3390/antiox9121175

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Journal: Antioxidants	Publication Date: Nov 25, 2020
Citations: 9	License type: CC BY 4.0

Affiliation: University of Granada

Abstract

Phenolic compounds have shown to have a high bioactive potential against various pathologies, postulating as an interesting alternative to manage some diseases. In this sense, both Lippia citriodora and Hibiscus sabdariffa are two botanical sources with a demonstrated high bioactive potential, in which their antioxidant capacity stands out. In this work, the optimization of the extraction conditions for the recovery of phytochemicals from L. citriodora leaves and H. sabdariffa calyces has been carried out using Response Surface Methodologies (RSM) considering their total polar compounds measured by HPLC-ESI-TOF/MS and Folin-Ciocalteu assay, and its antioxidant capacity evaluated by Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays. The results showed that to maximize the antioxidant capacity in H. sabdariffa, a moderate temperature and high ethanol percentage are needed, while a low temperature and a high percentage of ethanol are needed in L. citriodora. In addition, with the results obtained in the multiple response analysis, it is possible to affirm the importance of this type of analysis to develop functional ingredients, taking into account both total content of phenolic compounds and their bioactivity. Furthermore, as confirmed in this study, these analyses can be extrapolated in different techniques and in different matrices, with phenolic compounds from different families being important to develop new high added value products for food, pharmaceutical or cosmetic industries.

Highlights

In recent years, both nutraceuticals and functional food have received increasing attention from the scientific community, consumers and food manufacturers [1]
Attention can be focused on the composition of bioactive compounds as evaluating a wide group of compounds as polar compounds or more specific as phenolic compounds, since these are responsible for the vast majority of beneficial properties reported
In order to obtain reliable results in the multiple response analysis performed in H. sabdariffa, it was deemed appropriate to eliminate the response variable that the model had not adequately adjusted

Summary

Introduction

Both nutraceuticals and functional food have received increasing attention from the scientific community, consumers and food manufacturers [1]. This growing interest is related to their composition and health benefits. There is a huge variety of botanicals that have been reported to exhibit biological properties such as antioxidant or anti-inflammatory [2,3,4] In this sense, plants such as Hibiscus sabdariffa and Lippia citriodora stand out among an extensive list, since both have been traditionally used to prepare herbal drinks with numerous health benefits. Several of these beneficial properties have been related to the bioactive compounds present in their composition, mainly, phenolic compounds [5,6]

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