Abstract
BackgroundCommon bean (Phaseolus vulgaris L.) is an important warm-season food legume. Drought is the most important environmental stress factor affecting large areas of common bean via plant death or reduced global production. The NAM, ATAF1/2 and CUC2 (NAC) domain protein family are classic transcription factors (TFs) involved in a variety of abiotic stresses, particularly drought stress. However, the NAC TFs in common bean have not been characterized.ResultsIn the present study, 86 putative NAC TF proteins were identified from the common bean genome database and located on 11 common bean chromosomes. The proteins were phylogenetically clustered into 8 distinct subfamilies. The gene structure and motif composition of common bean NACs were similar in each subfamily. These results suggest that NACs in the same subfamily may possess conserved functions. The expression patterns of common bean NAC genes were also characterized. The majority of NACs exhibited specific temporal and spatial expression patterns. We identified 22 drought-related NAC TFs based on transcriptome data for drought-tolerant and drought-sensitive genotypes. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression patterns of the 20 drought-related NAC genes.ConclusionsBased on the common bean genome sequence, we analyzed the structural characteristics, genome distribution, and expression profiles of NAC gene family members and analyzed drought-responsive NAC genes. Our results provide useful information for the functional characterization of common bean NAC genes and rich resources and opportunities for understanding common bean drought stress tolerance mechanisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0882-5) contains supplementary material, which is available to authorized users.
Highlights
IntroductionDrought is the most important environmental stress factor affecting large areas of common bean via plant death or reduced global production
Common bean (Phaseolus vulgaris L.) is an important warm-season food legume
We identified 22 common bean NAC transcription factors (TFs) that were induced by drought stresses based on transcriptome data; these genes were of two types: differentially expressed between droughttolerant/sensitive genotypes and differentially expressed between treatment/control
Summary
Drought is the most important environmental stress factor affecting large areas of common bean via plant death or reduced global production. The NAM, ATAF1/2 and CUC2 (NAC) domain protein family are classic transcription factors (TFs) involved in a variety of abiotic stresses, drought stress. The growth and productivity of common bean are severely affected by abiotic stress, drought stress. Drought affects large areas of common bean in China by causing plant death or reducing production. The largest TF family is the bHLH family, which comprises 11,428 TFs, followed by MYB (8,746) and ERF (8,688). The species in this database represent Chlorophyta, Bryophyta, Lycopodiophyta, Coniferopsida, basal Magnoliophyta, Monocot and Eudicot. 10.9 % of the genome of the eudicot Glycine max encodes more than 5,069 TFs (3,714 loci) classified into 57 families [7]
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