Abstract
Semen Cuscutae is commonly used in traditional Chinese medicine and contains a series of compounds such as flavonoids, chlorogenic acids and lignans. In this study, we identified different kinds of compositions by ultra-high-performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (MS). A total of 45 compounds were observed, including 20 chlorogenic acids, 23 flavonoids and 2 lignans. 23 of them are reported for the first time including 6-O-caffeoyl-β-glucose, 3-O-(4′-O-Caffeoylglucosyl) quinic acid, etc. Their structures were established by retention behavior, extensive analyses of their MS spectra and further determined by comparison of their MS data with those reported in the literature. As chlorogenic acids and flavonoids are phenolic compounds that are predominant in Semen Cuscutae, in conclusion, phenolic compounds are the major constituents of Semen Cuscutae.
Highlights
Semen Cuscutae is the dry mature seed of Cuscuta australis R.Br. or Cuscuta chinensis Lam., belonging to convolvulaceae family
Cuscutae have been performed by HPLC-UV [5,6,7], but few studies have been performed by ultra-high-performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry
We identified 45 compositions in Semen Cuscutae using UPLC coupled with electrospray ionization tandem mass spectrometry system. 23 of them are reported for the first time including 6-O-caffeoyl-β-glucose, 3-O-(40 -O-Caffeoylglucosyl) quinic acid, etc
Summary
Semen Cuscutae is the dry mature seed of Cuscuta australis R.Br. or Cuscuta chinensis Lam., belonging to convolvulaceae family. Cuscutae have been performed by HPLC-UV [5,6,7], but few studies have been performed by ultra-high-performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry. This method has the advantage that it is more sensitive and selective than a HPLC-UV, leading to a more exact identification of a higher number of compounds [8]
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