Compound Specific Extraction of Camptothecin fromNothapodytes nimmonianaand Piperine fromPiper nigrumUsing Accelerated Solvent Extractor

Vinayak Upadhya,Harsha V Hegde,Ajay K Sharma,Sanjiva D Kholkute,Sandeep R Pai,Rajesh K Joshi

doi:10.1155/2014/932036

Abstract

Effects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solvent Extractor (ASE). Three cycles of extraction for a particular sample cell at a given temperature assured complete extraction. CPT and piperine were determined and quantified by using a simple and efficient UFLC-PDA (245 and 343 nm) method. Temperature increased efficiency of extraction to yield higher amount of CPT, whereas temperature had diminutive effect on yield of piperine. Maximum yield for CPT was achieved at 80°C and for piperine at 40°C. Thus, the study determines compound specific extraction of CPT from N. nimmoniana and piperine from P. nigrum using ASE method. The present study indicates the use of this method for simple, fast, and accurate extraction of the compound of interest.

Highlights

Camptothecin (CPT) a known potent anticancer active compound and piperine an economically important high valued alkaloid were used as the marker compounds (Figure 1(a))
Stem parts of N. nimmoniana and fruits of P. nigrum were collected from Belgaum (N 15.6383∘ E 074.2784∘) and North Canara (N 14.4721∘, E 074.5131∘), region of Western Ghats of Karnataka, India
The relative standard deviation (RSD) values for both analytes that were found less than 2% indicate that the methods used in this study were precised and reproducible

Summary

Introduction

Camptothecin (CPT) a known potent anticancer active compound and piperine an economically important high valued alkaloid were used as the marker compounds (Figure 1(a)). Camptothecin was originally isolated from a Chinese tree Camptotheca acuminata (Nyssaceae) [1]. It is reported in Nothapodytes nimmoniana and few other species belonging to unrelated orders of angiosperm classification [2,3,4]. Nothapodytes nimmoniana occupies important position in plant-based anticancer drugs because of CPT. Enormous demand for this alkaloid worldwide in the recent years has been subject to haphazard exploitation of the populations from wild. More than 20% decline in the population of N. nimmoniana from Western Ghats region has led to classify it in “vulnerable” category [5]

Methods

Results

Conclusion