Compositional Analysis of the Transgenic Potato with High-level Phytase Expression

Abstract

Phosphorus in the phytate form is undigestible by non-ruminant animals, causing environmental problems. The transgenic potato line 2-1 was generated with high phytase expression, and it could be a potential feed to improve phytate-P digestibility in non-ruminant animals. Compositional characteristics of genetically modified crops are one of key steps in safety assessment. To evaluate the feed safety of the transgenic potato line 2-1, the comparative analysis of compositions between the transgenic line 2-1 and the conventional line CK potato tubers for two consequent years was conducted. Only proline and zinc contents were shown to be significantly lower in both years, but within the database range. Trypsin inhibitors, lectins, and glycoalkaloids, including α-chaconines, and α-solanines, were also analyzed, and the contents had no significant differences between the two potato lines. The results suggested that the composition of the transgenic potato line 2-1 is substantially equivalent to that of the conventional line CK.