Composition of plant tissues and soil organic matter in the first stages of a vegetation succession

Abstract

Differences in soil organic matter (SOM) composition in consecutive soil profiles (under algae, grass, moss, heather and pine) from a primary vegetation succession (central Netherlands) were investigated by Cross Polarization-Magic Angle Spinning (CPMAS) 13C NMR, chemical degradation (acid hydrolysis of polysaccharides) and pyrolysis-GC/MS (Py-GC/MS) in relation to the vegetation. All mineral soil horizons had lower contents of polysaccharides than the ectorganic layer and/or the fresh plant material as suggested by the diminishing O-alkyl peaks by NMR. The monosaccharide distribution pointed to an increase of microbial sugars in the soil as suggested by the raising C 6/C 5 ratios. Soil polysaccharides may be accompanied by a significant contribution of carbohydrates from mosses in the first stages, because the undecomposed moss tissues have high C 6/C 5 ratios, which falsify the usual interpretation that such high ratios may be due to microbial sugars. Fresh litter and ectorganic layers under heather and pine had similar contents of aromatics, but the contribution of lignin (guaiacols and syringols) was lower in the ectorganic horizons. The degree of oxidation, as determined by the acid/aldehyde ratio, increased with progressive humification. All Ah horizons contained less aromatics, more oxidized lignin remnants and higher amounts of aliphatic compounds than the litter. One or two macromolecules, which produced n-alkenes/ n-alkanes and branched alkenes/alkanes upon pyrolysis and were derived from mosses, were mainly responsible for the accumulation of aliphatics in soil, although other aliphatic polymers, such as suberin, might also play an important role. Preservation of components from earlier vegetation might significantly influence SOM composition.