Abstract
Neutral and polar lipids in an extract of Pullularia pullulans were separated by a single silicic acid column. Of the total lipid content, 74% was found to be neutral lipids, on the average, and 26% was polar lipids. Gas chromatography of methyl esters of the fatty acids showed the presence of 15 acids, of which 59.2% were unsaturated. The most abundant unsaturated acid was C18 monounsaturated (41.9%) and the most abundant saturated acid wasC16 (30.8%). Gas chromatography and thin-layer chromatography revealed the presence of ergosterol, stigmasterol, and possibly squalene. Straight chain, branched chain, and saturated and unsaturated hydrocarbons were also found by gas chromatography. Trehalose was isolated and evidence suggests that it was esterified with one or more fatty acid residues.
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