Abstract
A cell line selection was carried out on sugarcane (Saccharum officinarum L.) callus, and the F. 156 (LT2) line having a high capability to produce lysine and threonine was isolated. This cell line contains more proteins, peptides and free amino acids than the control line. The amount and relative percentage of free and bound lysine and threonine were especially high in the LT2 line. In the free amino acid pool, the contents of the aspartic and glutamic acids of LT2 were also higher but that of alanine, valine, serine and proline tended to be lower than in the control. The biochemical modifications seem to be more important in the fractions of soluble proteins and free amino acids. This study showed the possibility of selection of overproducing amino acid cell lines in cereals and vegetable plants.Key words: Amino acids, lysine, threonine, cell line selection, sugarcane
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