Abstract
Plants harbor diverse communities of bacterial species in their internal compartments. Here we isolated and identified bacterial endophytes from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) to make working collection of endophytes and exploit their potentially beneficial properties toward plants and human being. A total of 1,886 bacteria were isolated from root, stem and leaf of MCGs grown in 24 different sites across the nation, using culture-dependent approach. Sequencing of 16S rDNA allowed us to classify them into 252 distinct groups. Taxonomic binning of them resulted in 117 operational taxonomic units (OTUs). Analysis of diversity indices across sampling sites and tissues suggested that composition of bacterial endophyte community within ginseng could differ substantially from one site to the next as well as from one host compartment to another. Assessment of 252 bacterial isolates for their beneficial traits to host plants showed that some bacteria possesses the ability to promote plant growth and produce ß-glucosidase, indicating their potential roles in plant growth promotion and bio-transformation. Taken together, our work provides not only valuable resources for utilization of bacterial endophytes in ginseng but also insights into bacterial communities inside a plant of medicinal importance.
Highlights
Plants harbor diverse communities of bacterial species in their internal compartments
A total of 1,886 culturable bacterial endophytes were initially isolated based on on-plate characteristics from 24 different sites and 3 different tissues of the ginseng plants to describe the culturable bacterial endophytes in MCGs and their potential properties (Fig. 1A and Supplementary Fig. S1)
One objective of this study was to examine the community composition of bacterial endophytes from MCG collected from 24 different sites
Summary
Plants harbor diverse communities of bacterial species in their internal compartments. We isolated and identified bacterial endophytes from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) to make working collection of endophytes and exploit their potentially beneficial properties toward plants and human being. Analysis of diversity indices across sampling sites and tissues suggested that composition of bacterial endophyte community within ginseng could differ substantially from one site to the as well as from one host compartment to another. Metagenomic studies employing next-generation sequencing technology are being increasingly used to discover and understand new bacterial endophytes of important food crops[12,13,14] These studies have led to identification of endophytic bacteria belonging to diverse taxa including Proteobacteria, Bacteriodetes, Firmicutes, and Actinobacteria. Successive cultivation in the same soil for a long time is known to be associated with changes in physicochemical properties of the soil, which in turn, frequently provide settings conducive to diseases by soil-borne pathogens, leading to devastating yield losses
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