Composition and In Vitro Study of Alocasia denudata Engler Stem Juice as a Wound Healing Agent

Abstract

Event Abstract Back to Event Composition and In Vitro Study of Alocasia denudata Engler Stem Juice as a Wound Healing Agent Siti Aisyah Arshad1, Asmah Hamid1, Farah Wahida Ibrahim1 and Mazlyzam Abdul Latif1* 1 National University of Malaysia, Biomedical Science Programme, Centre for Health & Applied Sciences, Faculty of Health Sciences, Malaysia Background The stem juice of Alocasia denudata Engler is known to possess wound healing properties. This study aimed to analyze the elemental composition and the presence of active compounds in A. denudata stem juice extract and to investigate in vitro wound healing regeneration of A. denudataon human dermal fibroblast cell lines. Methods The proximate element composition was assessed from freeze-dried sample of A. denudata stem juice extracts using scanning electron microscope (SEM). The active compounds of A. denudata stem juice were analyzed using Gas Chromatography-Mass Spectrometry (GCMS) and Liquid Chromatography-Mass Spectrometry (LCMS). The cytotoxicity of the juice was determined using MTT assay and the cell migration rate was determined by using scratch assay. Results Elemental screening showed the presence of oxygen, carbon, potassium, chlorine and sodium and GCMS analysis of the aqueous extract showed the presence of 9-octadecanamide (Z)-, octadecanoic acid-methyl ester, benzyl methyl disulphide as the major components. LCMS analysis revealed the presence of propanoic acid 2-hydroxy-, methyl ester as the major components. All the identified components listed are reported to be conducive for wound healing process. A. denudata extract was proven not cytotoxic to the cells and the highest cell viability was obtained at 62.5 μg/ml. The cells migration towards the wound area also increased compared to the control which shows the highest migration rate at 12-hour. The migration rate was 18 % higher compared to the control suggesting its potential in enhancing wound healing processes. Conclusion A. denudata stem juice extract has the potential to be developed as a wound healing agent. Acknowledgements This work was financially funded by Ministry of Education Fundamental Research Grant Scheme (FRGS/1/2014/SKK01/UKM/02/1) and Biomedical Science Programme, Centre for Health & Applied Sciences, Faculty of Health Sciences, UKM. Keywords: Alocasia denudata Engler, Wound Healing, fibroblast, active compounds, cell migration Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Miscellaneous Citation: Arshad S, Hamid A, Ibrahim F and Abdul Latif M (2019). Composition and In Vitro Study of Alocasia denudata Engler Stem Juice as a Wound Healing Agent. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00048 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Mazlyzam Abdul Latif, National University of Malaysia, Biomedical Science Programme, Centre for Health & Applied Sciences, Faculty of Health Sciences, Bangi, Malaysia, mazlyzam@ukm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Siti Aisyah Arshad Asmah Hamid Farah Wahida Ibrahim Mazlyzam Abdul Latif Google Siti Aisyah Arshad Asmah Hamid Farah Wahida Ibrahim Mazlyzam Abdul Latif Google Scholar Siti Aisyah Arshad Asmah Hamid Farah Wahida Ibrahim Mazlyzam Abdul Latif PubMed Siti Aisyah Arshad Asmah Hamid Farah Wahida Ibrahim Mazlyzam Abdul Latif Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.