Abstract
Kentucky bluegrass (Poa pratensis L.) cultivars are often blended to incorporate diverse characteristics. Factors that may contribute to the actual cultivar composition have not been evaluated. Through the use of DNA markers, individual plants in blended stands can be identified. This study evaluated changes in cultivar composition of `Blacksburg', `Midnight', and `Unique' Kentucky bluegrass blends. Characteristics such as seed size, seed moisture content, percent germination, and seedling development did not affect the initial composition of blends at time of seeding. DNA markers were used to demonstrate how the composition of a blended `Blacksburg', `Midnight', and `Unique' turf changed during the first growing season following establishment. The composition of blends did not significantly change from time of seeding in Sept. 1999 to Apr. 2000 or from Apr. 2000 to Oct. 2000. Two of the three blends were significantly different by Oct. 2000 relative to the percentages seeded in Sept. 1999.
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