Abstract
In present study, the anthocyanin composition and content of the fruit of B. heteropoda Schrenk were determined for the first time. The total anthocyanins were extracted from the fruit of B. heteropoda Schrenk using 0.5% HCl in 80% methanol and were then purified using an AB-8 macroporous resin column. The purified anthocyanin extract (PAE) was evaluated by high-performance liquid chromatography with a diode array detector (HPLC-DAD) and HPLC-high resolution-electrospray ionization-mass spectrometry (HPLC-HR-ESI-MS) under the same experimental conditions. The results revealed the presence of seven different anthocyanins. The major anthocyanins purified by preparative HPLC were confirmed to be delphinidin-3-O-glucopyranoside (30.3%), cyanidin-3-O-glucopyranoside (33.5%), petunidin-3-Ο-glucopyranoside (10.5%), peonidin-3-O-glucopyranoside (8.5%) and malvidin-3-O-glucopyranoside (13.8%) using HPLC-HR-ESI-MS and NMR spectroscopy. The total anthocyanin content was 2036.6 ± 2.2 mg/100 g of the fresh weight of B. heteropoda Schrenk fruit. In terms of its total reducing capacity assay, DPPH radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay and ABTS radical cation-scavenging activity assay, the PAE also showed potent antioxidant activity. The results are valuable for illuminating anthocyanins composition of B. heteropoda Schrenk and for further utilising them as a promising anthocyanin pigment source. This research enriched the chemical information of B. heteropoda Schrenk.
Highlights
Berberis heteropoda Schrenk, a type of Berberidaceae deciduous shrub that is native to the XinjiangUygur Autonomous Region of China, is used in both medicine and food [1]
The results showed that the reducing power of the purified anthocyanin extract (PAE) was approximately one-third that of ascorbic acid
Our research has provided the chemical basis for and evidence of high levels of antioxidant activity of the fruit of B. heteropoda Schrenk
Summary
Berberis heteropoda Schrenk, a type of Berberidaceae deciduous shrub that is native to the XinjiangUygur Autonomous Region of China, is used in both medicine and food [1]. The residents of Kazakh and Uighur have drunk tea made from it for a long time. The major anthocyanin constituents and the antioxidant activity of the fruit of B. heteropoda Schrenk have not yet been systematically studied, and in terms of large-scale applications, the potential of plant is basically untapped [2]. This situation has largely restricted the research and development of the fruit of B
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
