Abstract
YEAST polygalacturonase is an exo-cellular pectic enzyme produced by Saccharomyces fragilis in a synthetic, protein-free medium. The enzyme is constitutive and is not accompanied by pectinesterase1. When it is allowed to act on pectic acid, the following series of reactions occurs2: (a) Pectic acid → penta- + tetra- + tri- + digalacturonic acids; (b) Pentagalacturonic acid → tetra- + galacturonic acids; Pentagalacturonic acid → tri- + digalacturonic acids; (c) Tetragalacturonic acid → tri- + galacturonic acids; (d) Trigalacturonic acid → di- + galacturonic acids.
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