Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can be used to determine the distribution of single polymer species of non-ionic detergents of the polyoxyethylene type (Triton X-100 and 114, Tween 20 and Brij 35). Thin-layer chromatographic (TLC) and reversed-phase chromatographic (RPC) methods are presented which may separate single polymer species as verified by MALDI-MS. Comparison of chromatographic and MALDI-MS data show Poisson-like distributions for Triton X-100 without marked differences between different methods. Distribution parameters obtained with Triton X-100 charges from different suppliers are very similar. The RPC method used can be scaled up for preparation of pure detergent species.
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