Abstract
Different teas from everywhere are very useful and have been extensively studied. We studied the antioxidant activity of herbal teas and green teas from Hainan, Mallotus oblongifolius Muell. Arg. (MO), Ilex kudingcha C.J. Tseng (KD), Camellia sinensis var. assamica (J. W. Mast.) Kitam. Hainan Dayezhong (DY), and Camellia sinensis (L.) O. Ktze. (produced from Hainan Baisha (BS)). The total phenol content and total flavonoid content from water extracts, resin extracts and fractions of herbal teas and green teas were compared. Later, eight fractions of herbal teas and green teas were subjected to UPLC-PDA-ESI-(−)-HRMS. We determined 1-diphenyl -2-picryl-hydrazyl radical and hydroxyl free radical scavenging activity by electron paramagnetic resonance spectroscopy. We subjected Saccharomyces cerevisiae to hydrogen peroxide, stress and evaluated antioxidant activity of herbal teas and green teas in cellulo. The experiment identified more than 14 potential antioxidant compounds from herbal teas and green teas. The herbal teas and green teas had a clearance rate higher than ferulic acid at the same concentrations. MO best reduced intracellular oxidation levels and increased catalase, glutathione reductase activities, glutathione reduced and glutathione oxidized content. KD had the highest cell survival rate and reduced cell lipid peroxidation. DY best improved superoxide dismutase activity and BS was the most active in the halo test. Therefore, we concluded that MO had stronger antioxidant activity than other herbal teas and green teas from Hainan, especially, which reduce S. cerevisiae oxidative stress under H2O2 stress.
Highlights
Reactive oxygen species (ROS) are commonly produced as by-products of cellular metabolism, including superoxide anions (O2 ·− ), HO· and H2 O2
Four types of herbal teas and green teas purchased at Hainan Yesheng Food Co., Ltd. in Haikou, Hainan Province (China) in May 2017; MO was produced in Wanning, Hainan (China) in April 2017; kudingcha C.J. Tseng (KD) was produced in Wuzhishan, Hainan (China) in March 2017; DY was produced in Wuzhishan, Hainan (China) in March 2017 and BS was produced in Baisha, Hainan (China) in April 2017
We compared the antioxidant activities of herbal teas and green teas from Hainan, and identified that each herbal teas and green teas contained more than 14 antioxidant compounds
Summary
Reactive oxygen species (ROS) are commonly produced as by-products of cellular metabolism, including superoxide anions (O2 ·− ), HO· and H2 O2. Cells naturally have low levels of ROS that play significant roles in stimulating signaling pathways [1,2]. Excess ROS can arise from stressful environment or changes in oxidative metabolism of the cell, leading to oxidative stress, lipid peroxidation and cell damage [3]. Oxidative stress caused by excessive production of ROS is a key initiator of many chronic diseases [4]. Minimizing macromolecular damage caused by ROS can be an effective strategy to slow down aging and prevent age-related diseases such as cancer, Alzheimer’s disease and cardiovascular disease. Antioxidants can prevent ROS production [5,6]
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