Abstract
Wood, J. M. (University of Illinois, Urbana), and R. S. Wolfe. Components required for the formation of CH(4) from methylcobalamin by extracts of Methanobacillus omelianskii. J. Bacteriol. 92:696-700. 1966.-Optimal conditions for the formation of CH(4) from methylcobalamin (methyl-cobalt-5,6-dimethylbenzimidazolylcobamide) by partially purified extracts of Methanobacillus omelianskii (Methanobacterium omelianskii) were achieved by the addition of two protein fractions, adenosine triphosphate, Mg(++), and a reduced flavin adenine dinucleotide-generating system. Adenosine diphosphate and adenosine monophosphate play an important role in the regulation of C(1) transfer in this reaction. The possible position of adenosine triphosphate in B(12)-dependent methyl transfer is discussed.
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