Components of tissue fibrinolytic system as markers for processes in tumors of female reproductive organs.

Abstract

e17032 Background: Components of tissue fibrinolytic system are now considered as important agents providing progressive tumor growth. The purpose of the study was to analyze fibrinolytic system components in uterine and breast tumors. Methods: We studied tumor samples in breast cancer (BC, n=24), nodular type of fibrocystic breast disease (FBD, n-15) and uterine myoma (UM, n=14) and in synchronous BC and UM (n=12). Intact breast tissues (IBT, n=16) and intact myometrium (IM, n=14) were used as the control. Activity of plasmin (P), total content and activity of uPA-Ag and uPA-act, tPA-Ag and tPA-act were determined by ELISA; plasminogen (PG) concentration was measured by spectrophotometry (ACTICHROME PLG, USA). Results: uPA-Ag in solitary BC was 6.5 times higher and uPA-act – 4.5 times higher than in IBT. Concentration of tPA-Ag in 75% of BC patients exceeded the value in IBT by 2.1 times, and tPA-act in 87.5% – by 1.5 times. tPA-Ag and tPA-act in FBD tissues were similar to IBT values, while uPA-Ag and uPA-act were increased by 2.2 times each. uPA-Ag in BC in BC+UM exceeded the norm by 10.6 times, uPA-act – by 5.5 times; tPA-Ag in 75% of samples was 3.8 times higher than in IBT, and tPA-act – 2.7 times higher. uPA-Ag and uPA-act levels in UM in BC+UM were similar to IM values, while tPA-Ag and tPA-act exceeded the norm by 1.8 and 1.5 times, respectively. Both uPA and tPA levels and activity in solitary UM did not differ significantly from IM. Conclusions: The results show an active conversion of plasminogen into plasmin in both solitary and synchronous BC and in synchronous UM. Components of tissue fibrinolytic system can be considered as reliable markers for processes in solitary and synchronous tumors of female reproductive organs.