Abstract
The nuclear pore complex (NPC) is a multisubunit protein conglomerate that facilitates movement of RNA and protein between the nucleus and cytoplasm. Relatively little is known regarding the influence of the Arabidopsis NPC on growth and development. Seedling development, flowering time, nuclear morphology, mRNA accumulation, and gene expression changes in Arabidopsis nucleoporin mutants were investigated. Nuclear export of mRNA is differentially affected in plants with defects in nucleoporins that lie in different NPC subcomplexes. This study reveals differences in the manner by which nucleoporins alter molecular and plant growth phenotypes, suggesting that nuclear pore subcomplexes play distinct roles in nuclear transport and reveal a possible feedback relationship between the expression of genes involved in nuclear transport.
Highlights
The nuclear pore complex (NPC) is a massive macromolecular conglomerate that sits within invaginations of the nuclear envelope and controls nucleo-cytoplasmic transport of RNA and protein (Raices and D’Angelo, 2012)
In order to understand the phenotypic changes that are observed in nup160-4 and nup62-2 plants, an analysis of global gene expression was performed using the NASCarray service at the Nottingham Arabidopsis Stock Centre
Post- owering tpr1 mutants were compared with pre- owering wild-type plants and, more recently, early- owering 14-day-old hos1 was compared with wild-type plants (Jacob et al, 2007; MacGregor et al, 2013)
Summary
The nuclear pore complex (NPC) is a massive macromolecular conglomerate that sits within invaginations of the nuclear envelope and controls nucleo-cytoplasmic transport of RNA and protein (Raices and D’Angelo, 2012). NPC subcomplexes are comprised of individual nucleoporin (NUP) proteins These NUPs play roles in maintaining the structural integrity of the NPC (Walther et al, 2003), directly in uencing gene expression (Capelson et al, 2010; Kalverda et al, 2010; Van de Vosse et al, 2013), controlling differentiation (D’Angelo et al, 2012), maintaining regions of chromatin exclusion (Krull et al, 2010), or modulating nuclear transport (Walde and Kehlenbach, 2010). A growing portfolio of research in Drosophila (Capelson et al, 2010; Kalverda et al, 2010), Caenorhabditis elegans (Galy et al, 2003; Rodenas et al, 2012), ssion yeast (Bai et al, 2004), and trypanosomes (DeGrasse et al, 2009) indicates that general NPC and speci c NUP function may vary signi cantly between eukaryotes
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