Abstract

Taking the determination of main and minor components in ethanolic vanilla extracts and further commercial preparations as an example, it is investigated whether a method employing micellar electrokinetic chromatography (MEKC) can be regarded as a competitive alternative to already published methods employing high-performance liquid chromatography (HPLC) as separation method, if in both cases UV detection is employed. The developed MEKC method offered adequate resolution for all components included in this investigation within a much shorter analysis time (8 min run time + 4 min rinsing) than the HPLC alternative method (25 min run time +15 min rinsing and equilibration). The selectivities obtained with these two methods (peak elution orders) are orthogonal to each other with positive aspects concerning peak purity testing and peak identification. Limits of detection achieved with the MEKC method for solutions of standards are one to two orders of magnitude higher than those obtained with HPLC. However, limits of detection for real samples are also governed by resolution from minor and trace components. Results obtained with these two methods do not differ significantly for the compounds vanillin, vanillic acid, 4-hydroxybenzoic acid, and 4-hydroxybenzaldehyde, making MEKC an interesting alternative for the determination of these compounds in commercial preparations.

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