Complexity of tissue injury-induced nociceptive discharge of dorsal horn wide dynamic range neurons in the rat, correlation with the effect of systemic morphine

Abstract

Persistent discharge of wide dynamic range (WDR) neurons was recorded from lumbar dorsal horn of anesthetized rats following subcutaneous bee venom injection into the receptive field. To quantitatively describe the complexity of this nociceptive activity, we computed the approximate entropy (ApEn) for each sampled interspike interval (ISI) series. A larger value of ApEn indicates higher complexity or less regularity and vice versa. The ApEn value varied across different WDR neurons tested, and for each neuron the ApEn remained constant through the 1-h discharge though the average ISI of the sampled data increased progressively with time (16 neurons). A low dose of intravenous morphine (0.3 mg/kg) depressed the activity of WDR neurons differentially, and the degree of this inhibition showed a significant correlation with the value of ApEn ( P<0.001, 27 neurons, Spearman's correlation test). The present results suggest that the complexity feature of WDR neurons is various under tissue injury state, and for each single WDR neuron the complexity feature is relatively independent of the strength of peripheral noxious input and cannot be fully described in terms of average firing rate. Moreover, the response of the nociceptive discharge to analgesics may be related to the nonlinear dynamics feature of nociceptive neurons, which can be quantitatively characterized by the degree of complexity.