Complexity compounded: The evolution of phototransduction in stomatopods

Abstract

Event Abstract Back to Event Complexity compounded: The evolution of phototransduction in stomatopods Megan L. Porter1*, Daniel I. Speiser2, Alexander K. Zaharoff2, Roy L. Caldwell3, Todd H. Oakley2 and Thomas W. Cronin4 1 University of University Baltimore County, Biological Sciences, United States 2 University of California Santa Barbara, Ecology, Evolution, and Marine Biology, United States 3 University of California, Berkeley, Integrative Biology, United States 4 University of Maryland Baltimore County, Biological Sciences, United States Stomatopod crustaceans have complex visual systems containing up to 16 different spectral classes of photoreceptors. Complexity is also found at the molecular level, with a previous study of stomatopod opsin genes describing many more expressed transcripts than predicted based on photoreceptor physiology. How other phototransduction cascade components contribute to stomatopod visual system complexity is still unknown. This study continues research on stomatopod visual systems by investigating additional components of the G-protein coupled receptor (GPCR) phototransduction cascade using retinal transcriptome datasets (generated using 454 or Illumina platforms) from four species: Hemisquilla californiensis, Neogonodactylus oerstedii, Pseudosquilla ciliata, and Squilla empusa. These species represent taxonomic and visual system diversity within the order Stomatopoda. Using Drosophila melanogaster genes as queries, assembled transcriptomes from each species were searched for phototransduction transcripts using reciprocal BLAST methods. Based on these searches, transcripts from genes specifying 13 different phototransduction components were identified from stomatopod retinal transcriptomes; however, not all 13 genes were identified in any single species. Identified phototransduction genes include visual arrestins, all subunits of the heterotrimeric G-protein (i.e. Gαq, Gβ, Gγ), phospholipase C, G protein-coupled receptor kinases 1 and 2, transient receptor potential (TRP) channels, and opsin transcripts. The set of expressed transduction genes suggest that stomatopods utilize a Gq mediated GPCR signaling cascade in their retinal photoreceptors. Two of the phototransduction genes – arrestin and opsin – were identified in the assemblies of all four species. In particular, the opsin contigs identified expand known stomatopod diversity to short-wavelength and ultraviolet sensitive arthropod clades, and repeat the results of previous studies that recovered more opsin transcripts than spectrally distinct types of photoreceptors. Keywords: phototransduction, Stomatopoda, Opsin, GPCR signal transduction, visual system evolution, retinal transcriptomics Conference: International Conference on Invertebrate Vision, Fjälkinge, Sweden, 1 Aug - 8 Aug, 2013. Presentation Type: Oral presentation preferred Topic: Transduction, signalling and coding Citation: Porter ML, Speiser DI, Zaharoff AK, Caldwell RL, Oakley TH and Cronin TW (2019). Complexity compounded: The evolution of phototransduction in stomatopods. Front. Physiol. Conference Abstract: International Conference on Invertebrate Vision. doi: 10.3389/conf.fphys.2013.25.00010 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Feb 2013; Published Online: 09 Dec 2019. * Correspondence: Dr. Megan L Porter, University of University Baltimore County, Biological Sciences, Baltimore, MD, 21250, United States, Megan.Porter@usd.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Megan L Porter Daniel I Speiser Alexander K Zaharoff Roy L Caldwell Todd H Oakley Thomas W Cronin Google Megan L Porter Daniel I Speiser Alexander K Zaharoff Roy L Caldwell Todd H Oakley Thomas W Cronin Google Scholar Megan L Porter Daniel I Speiser Alexander K Zaharoff Roy L Caldwell Todd H Oakley Thomas W Cronin PubMed Megan L Porter Daniel I Speiser Alexander K Zaharoff Roy L Caldwell Todd H Oakley Thomas W Cronin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.