Abstract
β-lactamase profles of 38 inhibitor-resistant Escherichia coli isolates obtained from public hospitals in KwaZulu-Natal, selected on the basis of their resistance profles to one/more of amoxicillin/clavulanate, ampicillin/sulbactam and piperacillin/tazobactam were analysed. Isolates were subjected to iso-electric focusing, plasmid profle determination, PCR of the different β-lactamase genes and sequencing thereof to detect the possible mechanism/s of inhibitor-resistance. A range of β-lactamases including two inhibitor-resistant TEM β-lactamases (TEM-145 and TEM-146), a plasmid-mediated AmpC-type β-lactamase (CMY-20), OXA-1, TEM-55, SHV-2, CTX-M1 and TEM-1 was detected. Diverse β-lactamase genes and/or enzyme combinations, and plasmid profles inferred extensive mobilisation of resistance genes. Inhibitor resistance could be attributed to a range of mechanisms including but not limited to inhibitor-resistant TEM β-lactamases, hyper-production of TEM-1, hyper-production of chromosomal AmpC and OXA β-lactamases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Southern African Journal of Epidemiology and Infection
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
