Abstract
Summary The size, complexity, and complexity overlap of poly(A+) and poly(A−) mouse liver RNA fractions were investigated using RNA hybridization with either nonrepeated DNA or DNA complementary to nuclear RNA. The number average nucleotide size of poly(A+)hnRNA, poly(A+)mRNA, total mRNA, poly(A−)mRNA was 1750, 1500, 1460 and 1300 nucleotides respectively. The complexity of these populations in bases × 108 were nuclear RNA- 4.41, poly(A+)hnRNA- 2.68, poly(A−)hnRNA- 2.94, total mRNA- 0.71, poly(A+)mRNA- 0.79, poly(A−)mRNA- 0.72. The overlap in complexity between complex, infrequently copied poly(A+)mRNA and poly(A−)mRNA is essentially 100% thus indicating that they code for the same proteins.
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