Abstract
Phenobarbital-inducible cytochrome P450 variants have a higher affinity (app. K s = 0.6 μM) for metyrapone than do non-phenobarbital-inducible variants (app. K s = 80 μM). The difference in affinity has enabled us to quantitate, by metyrapone-induced difference spectral analysis, phenobarbital-inducible cytochromes P450 in liver microsomal membranes. The result obtained by this simple, rapid technique compare well with those found by more sophisticated techniques such as radioimmunoassay and immunoprecipitation.
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