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Abstract
ABSTRACT − To enhance therapeutic effects of insulin-sensitizing adipokine, ADN gene and potent agonists, rosiglitazonefor the PPARγ, cationic liposomes as non-viral vectors were formulated. The particle size and zeta potential of drug loadedand unloaded cationic liposomes were investigated. The complex formation between cationic liposomes and negativelycharged plasmid DNA was confirmed and the protection from DNase was observed. In vitro transfection was investigatedin HepG2, HeLa, and HEK293 cells by mRNA expression of ADN. Encapsulation efficacy of rosiglitazone-loaded lipo-somes was determined by UV detection. Particle sizes of cationic liposomes were in the range of 110-170 nm and thoseof rosiglitazone-loaded cationic liposomes were in the range of 130-180 nm, respectively. Gel retardation of complexes indi-cated that the complex was formed at weight ratios of cationic lipid to plasmid DNA higher than 20:1. Both complexes pro-tected plasmid DNA from DNase either drug free or drug loading. Encapsulation efficiency of rosiglitazone-loaded emulsionwas increased by drug dose. The mRNA expression levels of ADN were dose-dependently increased in cells transfectedwith plasmid DNA. Therefore, cationic liposomes could be potential co-delivery system for drug and gene. Key words
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