Abstract
Complex Variability within the THCA and CBDA Synthase Genes in Cannabis Species
Highlights
The history of marijuana (Cannabis sp.) use dates back thousands of years and there has been extensive analysis and characterization of the cannabinoid family of chemicals [1,2]
The tetrahydrocannabinolic acid (THCA) synthase gene(s) amplified from genomic DNA extracted from a total of 27 samples of seized marijuana from the Tulsa, Oklahoma area were sequenced on the Ion Torrent PGM platform
Cannabis species are known to manufacture a large family of highly related cannabinoid compounds, some of which are claimed to have medicinal effects ranging from stimulating appetite and easing anxiety for psychoactive cannabinoids like Δ-9-tetrahydrocannabinolic acid (THCA) to reducing the frequency and severity of seizures for the non-psychoactive cannabinoid cannabidiolic acid (CBDA) [12,13,14,15]
Summary
The history of marijuana (Cannabis sp.) use dates back thousands of years and there has been extensive analysis and characterization of the cannabinoid family of chemicals [1,2]. Simple molecular assays have been described that claim to be able to distinguish between active (active for catalysis) versus inactive (inactive for catalysis) synthase genes [4,6,7,8]. The rationale underlying such assays derives from SNPs that exist within both active and inactive genes that can be the targets for PCR primers that direct selective amplifications of active or inactive genes [6,7,8]. Inactive genes for THCA and CBDA synthase are likely to harbor many more SNP
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