Complex Interplay of Metabolic Pathways in Grafting of Ziziphus Species: Transcriptomic Insights into Regulatory Networks of Carbohydrates and Secondary Metabolite Biosynthesis

Abstract

Grafting serves as a pervasive methodology in the propagation of jujube plants, yet the nuanced molecular mechanisms that dictate rootstock-scion interactions remain inadequately understood. We examined the transcriptomic landscapes of jujube heterograft combinations. Contrary to self-grafting conditions, early-stage heterografting yielded no discernible advantageous effects on scion biomass accretion. Interestingly, the rootstock’s biomass was significantly impacted by the scion, varying by species. The differentially expressed genes (DEGs) across graft combinations were mainly enriched for the vegetative growth of rootstocks, secondary metabolism, and resistance improvement of scions. Weighted gene co-expression network analysis (WGCNA) identified 27 hub genes which were negatively correlated with plant growth and biomass enlargement, serving as negative regulators, while the genes, L484_001734, ATHB-15, and BPC1, were involved in positive regulation. With biomass measurements, the transcriptomic data supported that an incomplete vascular recovery during early grafting led to nutrient accumulation at the graft junction, temporarily limiting plant growth while providing development resources for callus. In summary, our work has demonstrated that the intricate biological connections between the rootstock and scion guarantee the effective jujube grafting process by elucidating the molecular processes involved in the process.