Complex gene regulation between young and old soybean leaves in responses to manganese toxicity

Abstract

Manganese (Mn) is an essential micronutrient for plant growth. However, excess manganese is toxic and inhibits crop production. Although it is widely known that physiological and molecular mechanisms underlie plant responses to Mn toxicity, few studies have been conducted to compare Mn tolerance capabilities between young and old leaves in plants; thus, the mechanisms underlying Mn tolerance in different plant tissues or organs are not fully understood. In this study, the dose responses of soybean to Mn availability were investigated. Genome-wide transcriptomic analysis was subsequently conducted to identify the differentially expressed genes (DEGs) in both young and old leaves of soybean in responses to Mn toxicity. Our results showed that excess Mn severely inhibited soybean growth and increased both Mn accumulation in and brown spots on soybean leaves, especially for the old leaves, strongly suggesting that more Mn was allocated to old leaves in soybean. Transcriptomic profiling revealed that totals of 4410 and 2258 DEGs were separately identified in young leaves and old leaves. Furthermore, only 944 DEGs were found to be commonly regulated in both young and old leaves of soybean, strongly suggesting distinct responses present in soybean young and old leaves in responses to Mn toxicity.