Complex formation of apo-enzyme, coenzyme and substrate of d-amino acid oxidase : III. Crystallization of artificial Michaelis complexes

Abstract

“Artificial Michaelis complexes” of d-amino acid oxidase and one or other of the “substrate-substitutes” benzoate and p-nitrobenzoate have been isolated in crystalline form. The complexes contain equimolecular amounts of apo-enzyme, FAD and substrate-substitute. The substrate-substitutes were driven off by an excess of d-alanine. The properties of the crystalline benzoate complex have been studied in detail.