Complex Formation between the Lumenal Domain of Adenovirus E3–19k Protein and the Extracellular Domain of Class I MHC Molecule In Vitro

Abstract

AbstractThe Ad2 E3–19k protein inhibits the transport of newly synthesized class I MHC molecules to the cell surface, thereby interfering with antigen presentation. The details of the interaction between E3–19k protein and class I MHC molecules have not been well‐defined. In this present study, we describe the use of gel filtration HPLC for confirming the binding interaction of two domain proteins, E3–19k and MHC class I antigen, and subsequently the characterization of protein complex by SDS‐PAGE. Our results demonstrate the complex formation between Ad2 lumenal E3–19k (108 amino acids, wt 108) and HLA‐A*0201 molecule in vitro. Titration experiments will be employed in the future to determine stoichiometry and verify the specific interactions.